Obtaining ImageJ
Download ImageJ from NIH
Using ImageJ
- Launch ImageJ
- Select the Line tool from the toolbar
- Select Set Measurements from the Analyze menu
- Deselect all boxes except Display Label
- Change Decimal Places to 2
- Select OK
- Draw a line along the ruler for some known interval (e.g., 1 cm)
- Select Set Scale from the Analyze menu
- Enter cm in the Unit of Length: box
- Select Global
- Select OK
- Draw a line across the character to be measured (hold the mouse button down from the start to the end of the measurement distance)
- Type command-M - a Results splash screen should appear with the sample number, Label (image name), Angle, and Length
- Save Data
- Select the Results screen
- Select Save As from the File menu
- Give the file a distinguishing name
- Save it to the desktop or Documents folder
Data Analysis
Go to R-Stat to learn how to obtain and use R.